Organelle Optogenetics: Direct Manipulation of Intracellular Ca²⁺ Dynamics by Light.

ABSTRACT: As one of the ubiquitous second messengers, the intracellular Ca²⁺, has been revealed to be a pivotal regulator of various cellular functions. Two major sources are involved in the initiation of Ca²⁺-dependent signals: influx from the extracellular space and release from the intracellular Ca²⁺ stores such as the endoplasmic/sarcoplasmic reticulum (ER/SR). To manipulate the Ca²⁺ release from the stores under high spatiotemporal precision, we established a new method termed "organelle optogenetics." That is, one of the light-sensitive cation channels (channelrhodopsin-green receiver, ChRGR), which is Ca²⁺-permeable, was specifically targeted to the ER/SR. The expression specificity as well as the functional operation of the ER/SR-targete

SUBMITTER: Asano T 

PROVIDER: S-EPMC6107701 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature