Long non-coding RNAs potentially function synergistically in the cellular reprogramming of SCNT embryos.

ABSTRACT:

Background

Long non-coding RNAs (lncRNAs), a type of epigenetic regulator, are thought to play important roles in embryonic development in mice, and several developmental defects are associated with epigenetic modification disorders. The most dramatic epigenetic reprogramming event occurs during somatic cell nuclear transfer (SCNT) when the expression profile of a differentiated cell is abolished, and a newly embryo-specific expression profile is established. However, the molecular mechanism underlying somatic reprogramming remains unclear, and the dynamics and functions of lncRNAs in this process have not yet been illustrated, resulting in inefficient reprogramming.

Results

In this study, 63 single-cell RNA-seq libraries were first generated and sequenced. A total of 7009 m

SUBMITTER: Wu F 

PROVIDER: S-EPMC6107955 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature