Nascent RNA sequencing analysis provides insights into enhancer-mediated gene regulation.

ABSTRACT:

Background

Enhancers are distal cis-regulatory elements that control gene expression. Despite an increasing appreciation of the importance of enhancers in cellular function and disease, our knowledge of enhancer-regulated transcription is very limited. Nascent RNA sequencing technologies, such as global nuclear run-on sequencing (GRO-seq) and precision run-on sequencing (PRO-seq), not only provide a direct and reliable measurement of enhancer activity, but also allow for quantifying transcription of enhancers and target genes simultaneously, making these technologies extremely useful for exploring enhancer-mediated regulation.

Results

Nascent RNA sequencing analysis (NRSA) provides a comprehensive view of enhancer-mediated gene regulation. NRSA not only outperforms existing

SUBMITTER: Wang J 

PROVIDER: S-EPMC6107967 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature