Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line.

ABSTRACT:

Background

Satellite cells are residential muscle stem cells that express a paired box protein, PAX7.

Results

Here, we report a knock-in mouse line expressing a PAX7-enhanced yellow fluorescent protein (YFP) fusion protein that enables visualization of PAX7 protein dynamics in living satellite cells through YFP fluorescence. The YFP fluorescence signals in Pax7-YFP knock-in mice clearly recapitulated the endogenous expression of PAX7 protein in satellite cells. YFP+ satellite cells were efficiently isolated from muscle tissues by fluorescence-activated cell sorting. Homozygous Pax7-YFP knock-in mice (Pax7^YFP/YFP) were viable, grew and regenerated muscle normally, and Pax7^YFP/YFP mouse-derived satellite cells proliferated, differentiated, and self-renewe

SUBMITTER: Kitajima Y 

PROVIDER: S-EPMC6108100 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature