Measurement of copy number variation in single cancer cells using rapid-emulsification digital droplet MDA.

ABSTRACT: Uniform amplification of low input DNA is important for applications across biology, including single-cell genomics, forensic science, and microbial and viral sequencing. However, the requisite biochemical amplification methods are prone to bias, skewing sequence proportions and obscuring signals relating to copy number. Digital droplet multiple displacement amplification enables uniform amplification, but requires expert knowledge of microfluidics to generate monodisperse emulsions. In addition, existing microfluidic methods are tedious and labor intensive for preparing many samples. Here, we introduce rapid emulsification multiple displacement amplification, a method to generate monodisperse droplets with a hand-held syringe and hierarchical droplet splitter. While conventional microflui

SUBMITTER: Kim SC 

PROVIDER: S-EPMC6108428 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature