Ontology highlight
ABSTRACT:
SUBMITTER: Song L
PROVIDER: S-EPMC6108506 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Song Letian L Ouedraogo Jean-Paul JP Kolbusz Magdalena M Nguyen Thi Truc Minh TTM Tsang Adrian A
PloS one 20180824 8
As a powerful tool for fast and precise genome editing, the CRISPR/Cas9 system has been applied in filamentous fungi to improve the efficiency of genome alteration. However, the method of delivering guide RNA (gRNA) remains a bottleneck in performing CRISPR mutagenesis in Aspergillus species. Here we report a gRNA transcription driven by endogenous tRNA promoters which include a tRNA gene plus 100 base pairs of upstream sequence. Co-transformation of a cas9-expressing plasmid with a linear DNA c ...[more]