Effects of FSS on the expression and localization of the core proteins in two Wnt signaling pathways, and their association with ciliogenesis.

ABSTRACT: Fluid shear stress (FSS) may alter ciliary structures and ciliogenesis, and it has been reported that the Wnt signaling pathway may regulate cilia assembly and disassembly. The present study aimed to investigate the effects of FSS on primary cilia, the Wnt/β‑catenin and Wnt/PCP signaling pathways, and the association among them. In the present study, human umbilical vein endothelial cells were subjected to FSS of differing velocities for various periods of time using a shear stress device. Subsequently, immunofluorescence and quantitative polymerase chain reaction were used to detect the expression and localization of the following core proteins: β‑catenin in the Wnt/β‑catenin signaling pathway; and dishevelled segment polarity protein 2 (Dvl2), fuzzy planar cell polarity protein (Fuz) and

SUBMITTER: Sheng X 

PROVIDER: S-EPMC6108851 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature