Collaboration of tRNA modifications and elongation factor eEF1A in decoding and nonsense suppression.

ABSTRACT: Transfer RNA (tRNA) from all domains of life contains multiple modified nucleosides, the functions of which remain incompletely understood. Genetic interactions between tRNA modification genes in Saccharomyces cerevisiae suggest that different tRNA modifications collaborate to maintain translational efficiency. Here we characterize such collaborative functions in the ochre suppressor tRNA SUP4. We quantified ochre read-through efficiency in mutants lacking either of the 7 known modifications in the extended anticodon stem loop (G26-C48). Absence of U34, U35, A37, U47 and C48 modifications partially impaired SUP4 function. We systematically combined modification defects and scored additive or synergistic negative effects on SUP4 performance. Our data reveal different degrees of functional r

SUBMITTER: Klassen R 

PROVIDER: S-EPMC6109124 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature