Transmembrane member 16A participates in hydrogen peroxide-induced apoptosis by facilitating mitochondria-dependent pathway in vascular smooth muscle cells.

ABSTRACT:

Background and purpose

Transmembrane member 16A (TMEM16A), an intrinsic constituent of the Ca²⁺ -activated Cl^- channel, is involved in vascular smooth muscle cell (VSMC) proliferation and hypertension-induced cerebrovascular remodelling. However, the functional significance of TMEM16A for apoptosis in basilar artery smooth muscle cells (BASMCs) remains elusive. Here, we investigated whether and how TMEM16A contributes to apoptosis in BASMCs.

Experimental approach

Cell viability assay, flow cytometry, Western blot, mitochondrial membrane potential assay, immunogold labelling and co-immunoprecipitation (co-IP) were performed.

Key results

Hydrogen peroxide (H₂ O₂ ) induced BASMC apoptosis through a mitochondria-dependent pathwa

SUBMITTER: Zeng JW 

PROVIDER: S-EPMC6109215 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature