Loss of Gαq impairs regulatory B-cell function.

ABSTRACT:

Background

Recent studies have shown a crucial role of Gαq in immune regulation, but how Gαq modulates regulatory B-cell (Breg) function is still unclear. We address this here.

Methods

CD19⁺IL-10⁺ Bregs of wild-type (WT) and Gnaq^-/- mice were analyzed by flow cytometry after stimulation by lipopolysaccharide. The WT and Gnaq^-/- Bregs were isolated and cocultured with WT CD4⁺CD25^- T cells in the presence of T-activator, and the proliferation of T cells and differentiation of regulatory T cells (Tregs) were analyzed by flow cytometry. We used inhibitors of PI3 kinase (PI3K), extracellular regulated protein kinases 1/2 (Erk1/2), and p38 mitogen-activated protein kinase (p38 MAPK) to detect the pathways involved i

SUBMITTER: He Y 

PROVIDER: S-EPMC6109260 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature