A GFP-fusion coupling FACS platform for advancing the metabolic engineering of filamentous fungi.

ABSTRACT:

Background

The filamentous fungus Trichoderma reesei, the most widely used cellulase producer, also has promising applications in lignocellulose-based biorefinery: consolidated bioprocessing for the production of high value-added products. However, such applications are thwarted by the time-consuming metabolic engineering processes (design-build-test-learn cycle) for T. reesei, resulted from (i) the spore separation-mediated purification as the multinucleate hyphae, (ii) transformant screening for high expression levels since unavailable of episomal expression system, and (iii) cases of inexpressible heterologous proteins.

Results

In this study, a GFP-fusion coupled fluorescence-activated cell sorting (FACS) platform was established to speed up the build and te

SUBMITTER: Wang G 

PROVIDER: S-EPMC6109270 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature