Improving agroinfiltration-based transient gene expression in Nicotiana benthamiana.

ABSTRACT:

Background

Agroinfiltration is a simple and effective method of delivering transgenes into plant cells for the rapid production of recombinant proteins and has become the preferred transient expression platform to manufacture biologics in plants. Despite its popularity, few studies have sought to improve the efficiency of agroinfiltration to further increase protein yields. This study aimed to increase agroinfiltration-based transient gene expression in Nicotiana benthamiana by improving all levels of transgenesis.

Results

Using the benchmark pEAQ-HT deconstructed virus vector system and the GUS reporter enzyme, physical, chemical, and molecular features were independently assessed for their ability to enhance Agrobacterium-mediated transformation and improve p

SUBMITTER: Norkunas K 

PROVIDER: S-EPMC6109318 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature