Cholesterol-enriched membrane microdomains are needed for insulin signaling and proliferation in hepatic cells.

ABSTRACT: Hepatocyte proliferation during liver regeneration is a well-coordinated process regulated by the activation of several growth factor receptors, including the insulin receptor (IR). The IR can be localized in part to cholesterol-enriched membrane microdomains, but the role of such domains in insulin-mediated events in hepatocytes is not known. We investigated whether partitioning of IRs into cholesterol-enriched membrane rafts is important for the mitogenic effects of insulin in the hepatic cells. IR and lipid rafts were labeled in HepG2 cells and primary rat hepatocytes. Membrane cholesterol was depleted in vitro with metyl-β-cyclodextrin (MβCD) and in vivo with lovastatin. Insulin-induced calcium (Ca²⁺) signals studies were examined in HepG2 cells and in freshly isolated rat h

SUBMITTER: Fonseca MC 

PROVIDER: S-EPMC6109708 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature