Comprehensive innate immune profiling of chikungunya virus infection in pediatric cases.

ABSTRACT: Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes global epidemics of debilitating disease worldwide. To gain functional insight into the host cellular genes required for virus infection, we performed whole-blood RNA-seq, 37-plex mass cytometry of peripheral blood mononuclear cells (PBMCs), and serum cytokine measurements of acute- and convalescent-phase samples obtained from 42 children naturally infected with CHIKV Semi-supervised classification and clustering of single-cell events into 57 sub-communities of canonical leukocyte phenotypes revealed a monocyte-driven response to acute infection, with the greatest expansions in "intermediate" CD14⁺⁺CD16⁺ monocytes and an activated subpopulation of CD14⁺ monocytes. Increases in acute-phase

SUBMITTER: Michlmayr D 

PROVIDER: S-EPMC6110311 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature