Single-cell mRNA profiling reveals the hierarchical response of miRNA targets to miRNA induction.

ABSTRACT: miRNAs are small RNAs that regulate gene expression post-transcriptionally. By repressing the translation and promoting the degradation of target mRNAs, miRNAs may reduce the cell-to-cell variability in protein expression, induce correlations between target expression levels, and provide a layer through which targets can influence each other's expression as "competing RNAs" (ceRNAs). However, experimental evidence for these behaviors is limited. Combining mathematical modeling with RNA sequencing of individual human embryonic kidney cells in which the expression of two distinct miRNAs was induced over a wide range, we have inferred parameters describing the response of hundreds of miRNA targets to miRNA induction. Individual targets have widely different response dynamics, and only a small

SUBMITTER: Rzepiela AJ 

PROVIDER: S-EPMC6110312 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature