Cation trafficking propels RNA hydrolysis.

ABSTRACT: Catalysis by members of the RNase H superfamily of enzymes is generally believed to require only two Mg²⁺ ions that are coordinated by active-site carboxylates. By examining the catalytic process of Bacillus halodurans RNase H1 in crystallo, however, we found that the two canonical Mg²⁺ ions and an additional K⁺ failed to align the nucleophilic water for RNA cleavage. Substrate alignment and product formation required a second K⁺ and a third Mg²⁺, which replaced the first K⁺ and departed immediately after cleavage. A third transient Mg²⁺ has also been observed for DNA synthesis, but in that case it coordinates the leaving group instead of the nucleophile as in the case of the RNase H1 hydrolysis reaction. These tr

SUBMITTER: Samara NL 

PROVIDER: S-EPMC6110950 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature