Data-independent proteome analysis of ARPE-19 cells.

ABSTRACT: We have performed a proteomics analysis of a human retinal pigment epithelial cell line (ARPE-19), which represents a widely used model for in vitro studies of cellular and molecular mechanisms related to human RPE cells (Dunn et al., 1996; Weigel et al., 2002) [1], [2]. Whole cell protein extracts were separated in four gel fractions via short (10 min) SDS-PAGE runs. Following fractionation and trypsin digestion, the resulting peptides were separated on a nano UPLC LC system and analyzed on-line with a QTof-IMS instrument: a tandem mass spectrometer with ion mobility separation (Synapt G2-Si). Data were acquired in data-independent mode (UDMS^E), which allows for absolute and/or relative post-acquisition protein quantification (Silva et al., 2006) [3]. The proteome profil

SUBMITTER: Koirala D 

PROVIDER: S-EPMC6111057 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature