Development and validation of a real-time PCR for the detection and quantification of porcine circovirus type 2.

ABSTRACT: Porcine circovirus type 2 (PCV2) is a spherical and non-enveloped virus belonging to the genus Circovirus of the Circoviridae family with a single stranded circular DNA genome. This virus, already detected worldwide, has been associated to several diseases and was implicated as the etiological agent of a disease named postweaning multisystemic wasting syndrome. Several methods have been described for the detection of PCV2, being real-time PCR the most simple and reliable. As far as we know, all the real-time PCR systems described until now are based on ORF2 gene, that exhibit the highest variability. This paper reports the development and validation of a real-time PCR targeted to ORF1 and based on a TaqMan probe for the detection of porcine circovirus type 2 DNA in swine samp

SUBMITTER: Henriques AM 

PROVIDER: S-EPMC6111954 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature