Characterisation of porcine epidemic diarrhea virus isolates during the 2014-2015 outbreak in the Philippines.

ABSTRACT: The viral agent of the porcine epidemic diarrhea (PED) was investigated during the reported 2014-2015 outbreaks in commercial farms in Central Luzon, Philippines. The study covered detection of PED virus (PEDV) in fecal and intestinal samples through reverse transcription PCR and sequence analysis of the nucleocapsid (N) gene. Results showed that 10 out of 34 fecal and intestinal samples examined were positive for PEDV. The partial nucleotide sequence of the N gene of the field samples showed 98-99% homologous to PEDV sequences registered in the GenBank. It was also noted that N gene sequences between field samples were 98% homologous. Interestingly, the partial sequences of the N genes of the field samples were genetically similar to the PEDV isolates from USA, China, Mexico, Canada and J

SUBMITTER: Garcia GG 

PROVIDER: S-EPMC6111962 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature