MiRge 2.0 for comprehensive analysis of microRNA sequencing data.

ABSTRACT:

Background

miRNAs play important roles in the regulation of gene expression. The rapidly developing field of microRNA sequencing (miRNA-seq; small RNA-seq) needs comprehensive, robust, user-friendly and standardized bioinformatics tools to analyze these large datasets. We present miRge 2.0, in which multiple enhancements were made towards these goals.

Results

miRge 2.0 has become more comprehensive with increased functionality including a novel miRNA detection method, A-to-I editing analysis, integrated standardized GFF3 isomiR reporting, and improved alignment to miRNAs. The novel miRNA detection method uniquely uses both miRNA hairpin sequence structure and composition of isomiRs resulting in higher specificity for potential miRNA identification. Using known miRNA data, ou

SUBMITTER: Lu Y 

PROVIDER: S-EPMC6112139 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature