Zebrafish Transcription Factor ORFeome for Gene Discovery and Regulatory Network Elucidation.

ABSTRACT: The completion of the zebrafish genome sequence and advances in miniaturization and multiplexing were essential to the creation of techniques such as RNA-seq, ChIP-seq, and high-throughput behavioral and chemical screens. Multiplexing was also instrumental in the recent enhancement of the classic yeast one-hybrid interaction techniques to provide unprecedented discovery capabilities for protein-DNA interactions. Unfortunately its use for zebrafish research is currently hampered by the lack of an open reading frame (ORF) clone collection. As a first step toward a complete collection, we describe a small library of transcriptional regulatory proteins comprising 142 ORFs and its potential applications.

SUBMITTER: King J 

PROVIDER: S-EPMC6112160 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature