Increased Ca⁺⁺ uptake by erythrocytes infected with malaria parasites: Evidence for exported proteins and novel inhibitors.

ABSTRACT: Malaria parasites export many proteins into their host erythrocytes and increase membrane permeability to diverse solutes. Although most solutes use a broad-selectivity channel known as the plasmodial surface anion channel, increased Ca⁺⁺ uptake is mediated by a distinct, poorly characterised mechanism that appears to be essential for the intracellular parasite. Here, we examined infected cell Ca⁺⁺ uptake with a kinetic fluorescence assay and the virulent human pathogen, Plasmodium falciparum. Cell surface labelling with N-hydroxysulfosuccinimide esters revealed differing effects on transport into infected and uninfected cells, indicating that Ca⁺⁺ uptake at the infected cell surface is mediated by new or altered proteins at the host membrane. Conditional k

SUBMITTER: Kushwaha AK 

PROVIDER: S-EPMC6113089 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature