Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid.

ABSTRACT: 13-cis-retinoic acid (isotretinoin, INN) is an oral pharmaceutical drug used for the treatment of skin acne, and is also a known teratogen. In this study, the molecular mechanisms underlying INN-induced developmental toxicity during early cardiac differentiation were investigated using both human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs). Pre-exposure of hiPSCs and hESCs to a sublethal concentration of INN did not influence cell proliferation and pluripotency. However, mesodermal differentiation was disrupted when INN was included in the medium during differentiation. Transcriptomic profiling by RNA-seq revealed that INN exposure leads to aberrant expression of genes involved in several signaling pathways that control early mesoderm differentiation, suc

SUBMITTER: Liu Q 

PROVIDER: S-EPMC6113333 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature