Calcium Oxalate Differentiates Human Monocytes Into Inflammatory M1 Macrophages.

ABSTRACT:

Purpose

A number of hyperoxaluric states have been associated with calcium oxalate (CaOx) deposits in the kidneys. In animal models of stone disease, these crystals interact with circulating monocytes that have migrated into the kidney as part of innate immunity. Similarly, macrophages surround CaOx crystals in kidneys of patients excreting high levels of oxalate. We investigate the effect of this exposure and subsequent human immunological response in vitro.

Materials and methods

Primary human monocytes were collected from healthy donors and exposed to CaOx, potassium oxalate, and zinc oxalate (ZnOx). Cytokine production was measured with a multiplex ELISA. Quantitative reverse transcription-polymerase chain reaction was done to validate the mRNA profile expression.

SUBMITTER: Dominguez-Gutierrez PR 

PROVIDER: S-EPMC6113402 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature