Evaluation of Two New Membrane-Based and Microtiter Plate Enzyme-Linked Immunosorbent Assays for Detection of Campylobacter jejuni in Stools of Bangladeshi Children.

ABSTRACT: Two new monoclonal antibody-based, sandwich enzyme immunoassays (EIAs) for fecal antigen detection of Campylobacter jejuni or Campylobacter coli were evaluated using diarrheal stool specimens from a cohort of children in Bangladesh. These children routinely harbor multiple enteric pathogens, often at levels that make it difficult to assign diarrheal symptoms to a causative agent. A panel of 158 PCR-positive specimens with a broad range of C. jejuni/C. coli DNA cycle threshold (C_T ) values was used to assess the ability of the two tests to detect C. jejuni/C. coli antigen amounts that varied widely. A panel of 100 C. jejuni/C. coli PCR-negative specimens was used to verify that the assays correctly identified specimen

SUBMITTER: Schnee AE 

PROVIDER: S-EPMC6113481 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature