Tumor-associated macrophages derived CCL18 promotes metastasis in squamous cell carcinoma of the head and neck.

ABSTRACT:

Background

Alternatively activated macrophages in tumor microenvironment is defined as M2 tumor-associated macrophages (M2 TAMs) that promote cancer progression. However, communicative mechanisms between M2 TAMs and cancer cells in squamous cell carcinoma of head and neck (SCCHN) remain largely unknown.

Methods

Quantitative real-time PCR, western blotting, enzyme-linked immunosorbent assay and flow cytometry were applied to quantify mRNA and protein expression of genes related to M2 TAMs, epithelial-mesenchymal transition (EMT) and stemness. Wounding-healing and Transwell invasion assays were performed to detect the invasion and migration. Sphere formation assay was used to detect the stemness of SCCHN cells. RNA-sequencing and following bioinformatics analysis were used to

SUBMITTER: She L 

PROVIDER: S-EPMC6114178 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature