Identification and validation of reference genes for qPCR in the terrestrial gastropod Cepaea nemoralis.

ABSTRACT: Identifying and understanding mechanisms that generate phenotypic diversity is a fundamental goal of evolutionary biology. With a diversity of pigmented shell morphotypes governed by Mendelian patterns of inheritance, the common grove snail Cepaea nemoralis (Linnaeus, 1758) has been a model for evolutionary biologists and population geneticists for decades. However, the genetic mechanisms by which C. nemoralis generates this pigmented shell diversity remain unknown. An important first step in investigating this pigmentation pattern is to establish a set of validated reference genes for differential gene expression assays. Here we have evaluated eleven candidate genes for reverse transcription quantitative polymerase chain reaction (qPCR) in C. nemoralis. Five of these were housekeeping gen

SUBMITTER: Affenzeller S 

PROVIDER: S-EPMC6114279 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature