Omega-3 and omega-6 fatty acid differentially impact cardiolipin remodeling in activated macrophage.

ABSTRACT:

Background

The macrophage plays an important role in innate immunity to induce immune responses. Lipid replacement therapy has been shown to change the lipid compositions of mitochondria and potentially becomes an alternative to reduce the inflammatory response.

Methods

We examined the effects of omega-6 arachidonic acid (AA), omega-3 eicosapentaenoic acid (EPA), and omega-3 docosahexaenoic acid (DHA) supplementation on the activated the macrophage cell line RAW264.7 via KdO₂-lipid A (KLA). The mitochondrial cardiolipin (CL) and monolysocardiolipin (MLCL) were analyzed by LC-MS.

Results

After macrophage activation by KLA, CL shifted to saturated species, but did not affect the quantity of CL. Inhibition of delta 6 desaturase also resulted in the same trend

SUBMITTER: Chang WH 

PROVIDER: S-EPMC6114728 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature