Divalent cation and chloride ion sites of chicken acid sensing ion channel 1a elucidated by x-ray crystallography.

ABSTRACT: Acid sensing ion channels (ASICs) are proton-gated ion channels that are members of the degenerin/epithelial sodium channel superfamily and are expressed throughout central and peripheral nervous systems. ASICs have been implicated in multiple physiological processes and are subject to numerous forms of endogenous and exogenous regulation that include modulation by Ca2+ and Cl- ions. However, the mapping of ion binding sites as well as a structure-based understanding of the mechanisms underlying ionic modulation of ASICs have remained elusive. Here we present ion binding sites of chicken ASIC1a in resting and desensitized states at high and low pH, respectively, determined by anomalous diffraction x-ray crystallography. The acidic pocket serves as a nexus for divalent cation binding at bot

SUBMITTER: Yoder N 

PROVIDER: S-EPMC6114778 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature