Exploring of the feature space of de novo developed post-transcriptional riboregulators.

ABSTRACT: Metabolic engineering increasingly depends upon RNA technology to customly rewire the metabolism to maximize production. To this end, pure riboregulators allow dynamic gene repression without the need of a potentially burdensome coexpressed protein like typical Hfq binding small RNAs and clustered regularly interspaced short palindromic repeats technology. Despite this clear advantage, no clear general design principles are available to de novo develop repressing riboregulators, limiting the availability and the reliable development of these type of riboregulators. Here, to overcome this lack of knowledge on the functionality of repressing riboregulators, translation inhibiting RNAs are developed from scratch. These de novo developed riboregulators explore features related to thermodynamic

SUBMITTER: Peters G 

PROVIDER: S-EPMC6114898 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature