DNA Repair Molecular Beacon assay: a platform for real-time functional analysis of cellular DNA repair capacity.

ABSTRACT: Numerous studies have shown that select DNA repair enzyme activities impact response and/or toxicity of genotoxins, suggesting a requirement for enzyme functional analyses to bolster precision medicine or prevention. To address this need, we developed a DNA Repair Molecular Beacon (DRMB) platform that rapidly measures DNA repair enzyme activity in real-time. The DRMB assay is applicable for discovery of DNA repair enzyme inhibitors, for the quantification of enzyme rates and is sufficiently sensitive to differentiate cellular enzymatic activity that stems from variation in expression or effects of amino acid substitutions. We show activity measures of several different base excision repair (BER) enzymes, including proteins with tumor-identified point mutations, revealing lesion-, lesion-co

SUBMITTER: Li J 

PROVIDER: S-EPMC6114979 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature