An Autoimmune Basis for Raynaud's Phenomenon: Murine Model and Human Disease.

ABSTRACT:

Objective

Raynaud's phenomenon (RP) is common in anti-RNP-positive patients with rheumatic diseases but is not itself known to be caused by autoimmunity. The aim of this study was to assess autoantibodies that could mediate this process.

Methods

Antibodies derived from patient sera and from murine models of anti-RNP autoimmunity were screened for the ability to induce RP-like tissue ischemia and endothelial cell apoptosis in murine models and in vitro systems.

Results

RNP-positive sera from RP patients and murine sera from RNP-positive B cell adoptive transfer recipients induced RP-like tissue ischemia and endothelial cell apoptosis. Proteomic analysis identified cytokeratin 10 (K10) as a candidate autoantigen in RP. Monoclonal anti-K10 antibodies reproduced patterns

SUBMITTER: Ascherman DP 

PROVIDER: S-EPMC6115278 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature