TERribly Difficult: Searching for Telomerase RNAs in Saccharomycetes.

ABSTRACT: The telomerase RNA in yeasts is large, usually >1000 nt, and contains functional elements that have been extensively studied experimentally in several disparate species. Nevertheless, they are very difficult to detect by homology-based methods and so far have escaped annotation in the majority of the genomes of Saccharomycotina. This is a consequence of sequences that evolve rapidly at nucleotide level, are subject to large variations in size, and are highly plastic with respect to their secondary structures. Here, we report on a survey that was aimed at closing this gap in RNA annotation. Despite considerable efforts and the combination of a variety of different methods, it was only partially successful. While 27 new telomerase RNAs were identified, we had to restrict our efforts to the s

SUBMITTER: Waldl M 

PROVIDER: S-EPMC6115765 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature