Genome-Wide Quantification of the Effect of Gene Overexpression on Escherichia coli Growth.

ABSTRACT: Recombinant protein production plays an essential role in both biological studies and pharmaceutical production. Escherichia coli is one of the most favorable hosts for this purpose. Although a number of strategies for optimizing protein production have been developed, the effect of gene overexpression on host cell growth has been much less studied. Here, we performed high-throughput tests on the E. coli a complete set of E. coli K-12 ORF archive (ASKA) collection to quantify the effects of overexpressing individual E. coli genes on its growth. The results indicated that overexpressing membrane-associated proteins or proteins with high abundances of branched-chain amino acids tended to impair cell growth, the latter of which could be remedied by amino acid suppl

SUBMITTER: Chen H 

PROVIDER: S-EPMC6116040 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature