Origins Left, Right, and Centre: Increasing the Number of Initiation Sites in the Escherichia coli Chromosome.

ABSTRACT: The bacterium Escherichia coli contains a single circular chromosome with a defined architecture. DNA replication initiates at a single origin called oriC. Two replication forks are assembled and proceed in opposite directions until they fuse in a specialised zone opposite the origin. This termination area is flanked by polar replication fork pause sites that allow forks to enter, but not to leave. Thus, the chromosome is divided into two replichores, each replicated by a single replication fork. Recently, we analysed the replication parameters in E. coli cells, in which an ectopic origin termed oriZ was integrated in the right-hand replichore. Two major obstacles to replication were identified: (1) head-on replication⁻transcription conflicts at highly transcrib

SUBMITTER: Dimude JU 

PROVIDER: S-EPMC6116050 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature