Spread of MCR-3 Colistin Resistance in China: An Epidemiological, Genomic and Mechanistic Study.

ABSTRACT:

Background

Mobilized resistance to colistin is evolving rapidly and its global dissemination poses a severe threat to human health and safety. Transferable colistin resistance gene, mcr-3, first identified in Shandong, China, has already been found in several countries in multidrug-resistant human infections. Here we track the spread of mcr-3 within 13 provinces in China and provide a complete characterization of its evolution, structure and function.

Methods

A total of 6497 non-duplicate samples were collected from thirteen provinces in China, from 2016 to 2017 and then screened for the presence of mcr-3 gene by PCR amplification. mcr-3-positive isolates were analyzed for antibiotic resistance and by southern blot hybridization, transfer analysis and plasmid typing. We then

SUBMITTER: Xu Y 

PROVIDER: S-EPMC6116419 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature