Diverse pathways of escape from all well-characterized VRC01-class broadly neutralizing HIV-1 antibodies.

ABSTRACT: Many broadly neutralizing antibodies (bNAbs) against human immunodeficiency virus type 1 (HIV-1) were shown effective in animal models, and are currently evaluated in clinical trials. However, use of these antibodies in humans is hampered by the rapid emergence of resistant viruses. Here we show that soft-randomization can be used to accelerate the parallel identification of viral escape pathways. As a proof of principle, we soft-randomized the epitope regions of VRC01-class bNAbs in replication-competent HIV-1 and selected for resistant variants. After only a few passages, a surprisingly diverse population of antibody-resistant viruses emerged, bearing both novel and previously described escape mutations. We observed that the escape variants resistant to some VRC01-class bNAbs are resista

SUBMITTER: Otsuka Y 

PROVIDER: S-EPMC6117093 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature