Detection of antibiotics synthetized in microfluidic picolitre-droplets by various actinobacteria.

ABSTRACT: The natural bacterial diversity is regarded as a treasure trove for natural products. However, accessing complex cell mixtures derived from environmental samples in standardized high-throughput screenings is challenging. Here, we present a droplet-based microfluidic platform for ultrahigh-throughput screenings able to directly harness the diversity of entire microbial communities. This platform combines extensive cultivation protocols in aqueous droplets starting from single cells or spores with modular detection methods for produced antimicrobial compounds. After long-term incubation for bacterial cell propagation and metabolite production, we implemented a setup for mass spectrometric analysis relying on direct electrospray ionization and injection of single droplets. Even in the presenc

SUBMITTER: Mahler L 

PROVIDER: S-EPMC6117260 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature