A fully defined static suspension culture system for large-scale human embryonic stem cell production.

ABSTRACT: Human embryonic stem cells (hESCs) play an important role in regenerative medicine due to their potential to differentiate into various functional cells. However, the conventional adherent culture system poses challenges to mass production of high-quality hESCs. Though scientists have made many attempts to establish a robust and economical hESC suspension culture system, there are existing limitations, including suboptimal passage methods and shear force caused by dynamic stirring. Here, we report on an efficient large-scale culture system, which enables long-term, GMP grade, single-cell inoculation, and serial expansion of hESCs with a yield of about 1.5 × 10⁹ cells per 1.5-L culture, while maintaining good pluripotency. The suspension culture system was enlarged gradually from

SUBMITTER: Li X 

PROVIDER: S-EPMC6117302 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature