DR region of Na⁺-K⁺-ATPase is a new target to protect heart against oxidative injury.

ABSTRACT: Previous studies have shown that the activity and expression of Na⁺/K⁺-ATPase (NKA) are down-regulated in the failing hearts, and that an antibody against the DR-region of NKA (DR-Ab) can stimulate its activity. The present study was designed to investigate the beneficial effects of this antibody against cardiac injury and the underlying mechanisms. We found that DR-Ab improved cardiac function, alleviated cardiac hypertrophy and reduced oxidative stress in isoproterenol-treated mice. In AC16 human cardiomyocytes, DR-Ab increased cell viability and attenuated apoptosis under oxidative stress. Corresponding to the observation of reduced NKA activity, NKA abundance on plasma membrane was lowered during oxidative stress. Suppressed activity of protein phosphatase 2 A (PP

SUBMITTER: Hua F 

PROVIDER: S-EPMC6117330 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature