Ontology highlight
ABSTRACT:
SUBMITTER: Guerillot R
PROVIDER: S-EPMC6117896 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Genome medicine 20180831 1
Mutation acquisition is a major mechanism of bacterial antibiotic resistance that remains insufficiently characterised. Here we present RM-seq, a new amplicon-based deep sequencing workflow based on a molecular barcoding technique adapted from Low Error Amplicon sequencing (LEA-seq). RM-seq allows detection and functional assessment of mutational resistance at high throughput from mixed bacterial populations. The sensitive detection of very low-frequency resistant sub-populations permits charact ...[more]