Influence of Micropatterned Grill Lines on Entamoeba histolytica Trophozoites Morphology and Migration.

ABSTRACT: Entamoeba histolytica, the causal agent of human amoebiasis, has two morphologically different phases: a resistant cyst and a trophozoite responsible for the invasion of the host tissues such as the colonic mucosa and the intestinal epithelium. During in vitro migration, trophozoites usually produce protuberances such as pseudopods and rarely filopodia, structures that have been observed in the interaction of trophozoites with human colonic epithelial tissue. To study the different membrane projections produced by the trophozoites, including pseudopods, filopodia, uropods, blebs, and others, we designed an induction system using erythrocyte extract or fibronectin (FN) in micropatterned grill lines (each micro-line containing multiple micro-portions of FN or erythrocyte extrac

SUBMITTER: Sierra-Lopez F 

PROVIDER: S-EPMC6117912 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature