Neutralizing antibodies against porcine epidemic diarrhea virus block virus attachment and internalization.

ABSTRACT:

Background

Porcine epidemic diarrhea virus (PEDV) is emerging as a pathogenic coronavirus that causes a huge economic burden to the swine industry. Interaction of the viral spike (S) surface glycoprotein with the host cell receptor is recognized as the first step of infection and is the main determinant of virus tropism. The mechanisms by which neutralizing antibodies inhibit PEDV have not been defined. Isolating PEDV neutralizing antibodies are crucial to identifying the receptor-binding domains of the viral spike and elucidating the mechanism of protection against PEDV infection.

Methods

B cell hybridoma technique was used to generate hybridoma cells that secrete specific antibodies. E.coli prokaryotic expression system and Bac-to-Bac expression system were used to identif

SUBMITTER: Gong L 

PROVIDER: S-EPMC6117962 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature