A perfused human blood-brain barrier on-a-chip for high-throughput assessment of barrier function and antibody transport.

ABSTRACT:

Background

Receptor-mediated transcytosis is one of the major routes for drug delivery of large molecules into the brain. The aim of this study was to develop a novel model of the human blood-brain barrier (BBB) in a high-throughput microfluidic device. This model can be used to assess passage of large biopharmaceuticals, such as therapeutic antibodies, across the BBB.

Methods

The model comprises human cell lines of brain endothelial cells, astrocytes, and pericytes in a two-lane or three-lane microfluidic platform that harbors 96 or 40 chips, respectively, in a 384-well plate format. In each chip, a perfused vessel of brain endothelial cells was grown against an extracellular matrix gel, which was patterned by means of surface tension techniques. Astrocytes and pericytes we

SUBMITTER: Wevers NR 

PROVIDER: S-EPMC6117964 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature