Perturbed maintenance of transcriptional repression on the inactive X-chromosome in the mouse brain after Xist deletion.

ABSTRACT:

Background

The long noncoding RNA Xist is critical for initiation and establishment of X-chromosome inactivation during embryogenesis in mammals, but it is unclear whether its continued expression is required for maintaining X-inactivation in vivo.

Results

By using an inactive X-chromosome-linked MeCP2-GFP reporter, which allowed us to enumerate reactivation events in the mouse brain even when they occur in very few cells, we found that deletion of Xist in the brain after establishment of X-chromosome inactivation leads to reactivation in 2-5% of neurons and in a smaller fraction of astrocytes. In contrast to global loss of both H3 lysine 27 trimethylation (H3K27m3) and histone H2A lysine 119 monoubiquitylation (H2AK119ub1) we observed upon Xist deletion, alterations in CpG

SUBMITTER: Adrianse RL 

PROVIDER: S-EPMC6118007 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature