ITRAQ-based proteomics profiling of Schwann cells before and after peripheral nerve injury.

ABSTRACT:

Objectives

Schwann cells (SCs) have a wide range of applications as seed cells in the treatment of nerve injury during transplantation. However, there has been no report yet on kinds of proteomics changes that occur in Schwann cells before and after peripheral nerve injury.

Materials and methods

Activated Schwann cells (ASCs) and normal Schwann cells (NSCs) were obtained from adult Wistar rat sciatic nerves. After immunofluorescence identification, we identified differentially expressed proteins in the ASCs and NSCs using isobaric tags for relative and absolute quantitation (iTRAQ) combined with high-resolution Orbitrap liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). In addition, all the differentially expressed proteins were analyzed by Gene ontology (

SUBMITTER: Shi GD 

PROVIDER: S-EPMC6118081 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature