Characterizing the multiplicity of HIV founder variants during sexual transmission among MSM.

ABSTRACT: Transmission of multiple founder variants has been associated with faster HIV disease progression. Many studies have attempted to determine the number of founder variants, mainly by analysis of sequence diversity and/or tree topology from acutely HIV-infected individuals. We hypothesized that adding sequence data collected from source partners might improve resolution and characterization of transmission events. Blood plasma samples were collected from both the source and recipient in thirty epidemiologically- and phylogenetically linked transmission pairs. All were men who have sex with men, sampled on average 70 days (range 11-170) after the recipient's estimated date of infection. Next generation sequencing (454 FLX, Roche) of HIV-1 env (C2-V3) was performed for all samples. Insp

SUBMITTER: Chaillon A 

PROVIDER: S-EPMC6118227 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature