Mass Spectrometry-Based Chemical and Enzymatic Methods for Global Analysis of Protein Glycosylation.

ABSTRACT: Glycosylation is one of the most common protein modifications, and it is essential for mammalian cell survival. It often determines protein folding and trafficking, and regulates nearly every extracellular activity, including cell-cell communication and cell-matrix interactions. Aberrant protein glycosylation events are hallmarks of human diseases such as cancer and infectious diseases. Therefore, glycoproteins can serve as effective biomarkers for disease detection and targets for drug and vaccine development. Despite the importance of glycoproteins, global analysis of protein glycosylation (either glycoproteins or glycans) in complex biological samples has been a daunting task, and here we mainly focus on glycoprotein analysis using mass spectrometry (MS)-based bottom-up proteomics. Alth

SUBMITTER: Xiao H 

PROVIDER: S-EPMC6118346 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature