Esophageal IgG4 levels correlate with histopathologic and transcriptomic features in eosinophilic esophagitis.

ABSTRACT:

Background

Recent data associate eosinophilic esophagitis (EoE) with IgG4 rather than IgE, but its significance and function have not been determined. Our aims were to measure esophageal IgG4 levels and to determine functional correlations as assessed by histologic and transcriptome analyses.

Methods

This case-control study included pediatric subjects with EoE (≥15 eosinophils/HPF) and non-EoE controls. Protein lysates were analyzed for IgA, IgM, and IgG1-IgG4 using the Luminex 100 system; IgE was quantified by ELISA. Esophageal biopsies were scored using the EoE histology scoring system. Transcripts were probed by the EoE diagnostic panel, designed to examine the expression of 96 esophageal transcripts.

Results

Esophageal IgG subclasses, IgA, and IgM, but not IgE, we

SUBMITTER: Rosenberg CE 

PROVIDER: S-EPMC6119084 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature