Analysis of lipid adsorption on nanoparticles by nanoflow liquid chromatography-tandem mass spectrometry.

ABSTRACT: Nanoparticles (NPs) tend to adsorb matrix molecules like proteins and lipids incubated with biological fluids, forming a biological corona. While the formation and functions of protein corona have been studied extensively, little attention has been paid to lipid adsorption on NPs. However, lipids are also abundantly present in biological fluids and play important roles in processes like cell signaling and angiogenesis. Therefore, in this study, we established the analytical procedure for study of lipid adsorption on three different types of NPs in two matrices: human serum and heavy cream, using nanoflow liquid chromatography-mass spectrometry (nanoflowLC-MS). Serum was chosen to represent the common environment the NPs would be present once entering human body, and heavy cream was the rep

SUBMITTER: Lee JY 

PROVIDER: S-EPMC6119100 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature